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KMID : 0364819850230040271
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Korean Journal of Microbiology
1985 Volume.23 No. 4 p.271 ~ p.281
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Purification and Partial Characterization of Bacillus thuringiensis var. thuringiensis Exotoxin
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Abstract
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1
Bacillus thuringiensis var. thuringiensis produces an extracellular insecticidal thermostable 8-exotoxin, which was purified through microfiltering, barium precipitation, charcoal absorption chromatography, ion exchange column chromatography and gel filtration. The exotoxin in each purification step was detected by thin layer chromatography, high pressure liquid chromatography and paper electrophoresis with efficient results.
The exotoxin productivity on time course was checked by spectrophotometric absorbance at 258 nm with the result that the exotoxin was initially produced in 6 hour culture and reached maximum
value in 36 hour culture. Anti-bacterial effect test on Micrococcusflava was applied as toxicity test. The
results showed that growth inhibition of M. (lava could be shown in plate assay of cell free filtered supernatant, alkaline eluant from charcoal and purified exotoxin obtained from gel filtration column. The estimated molecular weight of the exotoxin by gel filtration column chromatography on Sephadex G-10 appeared to be 740. Heat stability of the exotoxin was confirmed through autoclaving twice.
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